LOCUS       Exported                3924 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0042max_regulator_rhlR
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Daniel Gerngross
  TITLE     piG0042max
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..95
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        139..173
                     /note="const. promoter (J23100)"
     RBS             180..213
                     /note="Salis RBS Calculator, TIR=1541765.04"
     CDS             214..942
                     /codon_start=1
                     /product="RhlR (no LVA)"
                     /note="rhlR (C0171)"
                     /translation="MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAY
                     GVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQS
                     RMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLT
                     QKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKK
                     FDAPNKTLAAAYAAALGLI*"
     terminator      951..1079
                     /note="directional double term (B0015)"
     terminator      1091..1177
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1359..1550
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1652..1792
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1963..2582)
                     /direction=LEFT
                     /note="pBR322_origin"
     oriT            2602..2710
                     /note="incP origin of transfer"
     CDS             complement(2900..3760)
                     /note="AmpR"
     promoter        complement(3761..3852)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaaga ctagtcgcca gggttttccc
      121 agtcacgacg cggccgcatt gacggctagc tcagtcctag gtacagtgct agctactaga
      181 taaaagacgt aataagatat aaggaggtta ataatgagga atgacggagg ctttttgctg
      241 tggtgggacg gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc
      301 gtcctggaaa aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac
      361 acgattccct tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg
      421 gagcgatacc agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc
      481 tcctcggaaa tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac
      541 gaggctcgcg attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat
      601 ttgctcagcg tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag
      661 gaaatccgcc tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg
      721 gagcatccga tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg
      781 caatggaccg ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag
      841 agcacggtga acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg
      901 ctggctgccg cctacgccgc ggcgctgggt ctcatctaat aatactagag ccaggcatca
      961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1021 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttataa
     1081 ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1141 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cagctgggcg
     1201 cgcccccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     1261 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     1321 cccatgaaca gaaattcccc cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg
     1381 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     1441 agctggacgc ggatgaacag gcagacatct gtgaatcgct gcacgaccac gctgatgagc
     1501 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1561 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1621 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     1681 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     1741 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     1801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2401 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2581 cttttctacg gggccggcct acggccagcc tcgcagagca ggattcccgt tgagcaccgc
     2641 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     2701 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     2761 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     2821 gaagcagggt tatgcagcgg aaaaggacaa gggtcgtcca aaaaaaaagg ctccaaaagg
     2881 agcctttaat tgtatcggtt taccaatgtt taatcaggct cgcgccaatt tccgcaatct
     2941 gacggttacg ttcatccatc gtcgcctggc tgccggtggt ataaatcacc acaatacggc
     3001 tcggtttacc atccgggccc agcgccgcaa taatgccacg gctgccacgt tcgcccgcac
     3061 cgcttttatc cgcaataaac cagccagccg gcagcgcgct acgcagcagc ggacccgcca
     3121 ctttatccgc ttccatccaa tcaatcagtt gctggcggct tgccagggtc agcagctcac
     3181 ccgtcagcag tttacgcagg gtggtcgcca ttgctgccgg catggtggta tcacgttcat
     3241 cgttcggaat cgcttcgttc agttccggtt cccaacgatc cagacgggtc acatgatcgc
     3301 ccatgttatg cagaaacgcg gtcagttctt tcggaccgcc aatggtcgtc agcagcaggt
     3361 tcgccgcggt gttatcgctc atggtaatcg ccgcgctgca cagttcacgc acggtcatgc
     3421 catcggtcag atgtttttcg gtcaccgggc tatattccac cagatcgttc tggctataat
     3481 gaatacgacg gcccagttgt tcctggcccg catccacacg gctcagaacc gcaccgcaca
     3541 gcagcacttt aaaggtgctc atcatcggaa aacgttcttc cggacgaaaa gattccagaa
     3601 ttttgccgct gttcagatcc agttcaatat agcccacgcg cgcacccagt tgatcttccg
     3661 catctttcac tttcaccagg gtttccggat gcgcaaacac cggcaggcaa aacgccgcaa
     3721 aaaacggaat cagcgccaca cgaaaatgct gaatgctcat actcttcctt tttcaatatt
     3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3841 aaaataaaca aataggggaa ttaaaaaaaa gcccgctcat taggcgggct actaatttaa
     3901 atcgtaatta ttggggaccc ctgg
//
